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I am not a Bruker employee but I work for my PhD in the field of mass spectrometry, so let me try to answer your questions. qToFs are typically coupled with ESI (or other API sources) because the ToF measurement requires a defined starting point. In case of MALDI, this starting point is defined by each laser pulse and, hence, MALDI is directly coupled with ToF analyzers. In case of ESI, the starting point is defined by an orthogonal accelerator (oaToF) that accelerates the ions orthogonal to their original flight path. This oaToF set-up allows for coupling with quadrupoles as the orthogonal accelerator requires low ion speeds, which are also required for quadrupoles. The MALDI ToF coupling, on the other hand, provides extremely fast moving ions which prevents the usage of quadrupoles in these devices. Answering the question about dis/advantages of qToF vs ToF/ToF is hard because both techniques are well suited for acquiring MS/MS spectra. Overall, its rather a question of which ionization technique is more suitable for your samples. However, I personally prefer the qToF set-up for MS/MS experiments because quadrupole collision cells allow for measurements at well defined collision energies which are really important for my research.

Dear Abhay Pandey, the LODs and LOQs are highly dependent on the analytes selected, matrix analyzed and method used. For example, you can achieve sub-ppb LODs for most of the pesticides in fruits and vegetables matrices using QuEChERS sample preparation or sub-ppt LODs and LOQ for PFAS in water samples using direct injection.

Sir, as I am working on developing method for Quantitation of antibiotic residues in milk and i have bruker lc tqd. I wanted to know that what can be the LOD for methods. Can I go till ppt level with this instrument (lc ms evoq elite ER. Thanking you sir

@@abhaypandey9822 thank you for being so interested in our products :) We would love to start a deeper discussion with you. Would you mind getting in contact with Mass.bdal.es@bruker.com or esi.appl.support@Bruker.com

You find it irritating because you are not German resident. After one week in Berlin I think you will like the background music.

Hi Paul, thank you for reaching out. In this case could you please contact our software support? Just let us know if you need the contact details. Kind regards Bruker

@@BrukerDaltonics thanks, your Support helped me out a lot! Is there a part 3 to this series?

Hi Samir, thank you for your request. Would you like to share your contact details with us? For a detailed answer we would like to hand over your question to one of our product experts. Kind regards, the Bruker Marketing Team

Hi Erwin, thank you for your question. The microflex LT/SH mass spectrometer uses also the MALDI Methode (Matrix Assisted Laser Desorption Ionization).

Hi Stefunny, thanks for your question. TOF stands for “Time Of Flight”. TOF TOF means “Tandem Time Of Flight”. Both Mass Spectrometry concepts are similar but different during the measurement of your sample. MALDI TOF makes it possible to determine molecular masses while MALDI TOF-TOF provides you an deeper analytical insight of targeted compounds.

Hi Study Group, our Team used mainly "Cinema 4D" for the 3D Animation. For Video editing we used "Adobe Premiere" and "Adobe After Effects".

@@BrukerDaltonics thanks a lot sir for replying (•‿•)

Hi chachouromynews , in this Video we are showing our "rapifleX". This Instrument has a size of 95 x 80 x 275 cm. Of course we also have smaller Bench Top Instruments, like our Microflex Series.

We are glad you liked it, the Song is called "Breaking News" by Goodstreet

No, the MALDI Biotyper can identify up to species level. For strain typing we recommend using our IR Biotyper. You can check our product Page for further Information: www.bruker.com/en/applications/microbiology-and-diagnostics/food-beverage-microbiology/ir-biotyper-for-food-microbiology.html

Hi Hao Zhang, thank you for your Interest and your feedback. We are explaining the PASEF principle in one of our other Videos: ruclips.net/video/J0uGhyVDiuQ/видео.html You can have a look at Minute 01:00 - 01:50, maybe this helps a little bit to visualize the process. :)